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Once you get specific enough with your assumptions, then you can devise a plan to test (de-risk) them. The most important assumptions to de-risk first are the ones that are necessary conditions for success and that you are most uncertain about. For example, in the startup context, take the assumption that your solution sufficiently solves the problem it was designed to solve. If this assumption is untrue, then you will need to change what you are doing immediately before you can proceed any further, because the whole endeavor won't work otherwise.